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KMID : 0379520030190040285
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한국독성학회지
2003 Volume.19 No. 4 p.285 ~ p.291
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Evaluation of Skin Sensitization Potential of Skin Whitening Agents by Local Lymph Node Assay
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Lee Jong-Kwon
Park Seung-Hee
Park Jae-Hyun
Kim Hyung-Soo
Eom Joon-Ho
Nam Ki-Taek
Han Beom-Seok
Jang Dong-Deuk
Choi Kwang-Sik
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Abstract
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The use of skin whitening agents has been recently increased in various kinds of cosmetic products, although there were some reports that whitening agents might cause allergic contact dermatitis. A murine local lymph node assay (LLNA) has been developed as an alternative to guinea pigs for contact sensitization potential. This study was carried out to investigate the skin sensitization potential of three whitening agents, arbutin, azelaic acid, and kojic acid, by LLNA using a non-radiois-topic endpoint. Female Balb/c mice were exposed topically to a weak allergen, alpha-hexylcinnamalde-hyde (HCA), and three whitening agents following LLNA protocol. Lymph node (LN) weight and cell proliferation in ears and auricular lymph node using bromodeoxyuridine (BrdU) immunohistochemistry were evaluated. LN weights were significantly increased at the HCA group compared to the vehicle control. A weak allergen, HCA elicited 3-fold or greater increase in cell proliferation of lymph nodes as well as increase in cell proliferation of ear as measured by BrdU immunohistochemistry. However, in the case of skin whitening agent groups, there were no significant changes in LN weight and cell proliferation in the ear and lymph node of mice treated with 5, 10 and 20% of three whitening agents compared to the vehicle control. These results show that these three skin whitening agents may not have contact sensitization potentials at tested concentrations in Balb/c mice by LLNA.
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KEYWORD
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Skin whitening agents, BrdU, Immunohistochemistry, Local lymph node assay
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